Effects of si<i>Ing3</i> injection on the levels of AcH4K12, asymmetric cell division–related gene expression, and mTOR protein in mouse oocytes.

<p>(A) The levels of AcH4K12 in fully grown GV oocytes cultured for 15.5 h after injection of si<i>Ing3</i> were significantly decreased as compared to controls. Red, AcH4K12; blue, chromatin. (B) <i>mTOR</i> mRNA levels were significantly decreased after si<i>Ing3</i> injection in fully grown GV oocytes (*<i>p</i><0.05) as compared with other asymmetric cell division–related genes. (C) Although the protein localization of mTOR was not changed, the amount of mTOR protein was reduced in si<i>Ing3</i>-injected oocytes. Green, mTOR; blue, chromatin. (D) The expression levels of mTOR-downstream genes, such as <i>Cdc42</i>, <i>Rac1</i>, and <i>RhoA,</i> in fully grown GV oocytes were significantly decreased 15.5 h after si<i>Ing3</i> injection (*<i>p</i><0.05).</p

Kaplan–Meier analysis of cumulative survival.

<p>Kaplan–Meier analysis of cumulative survival.</p

Effects of si<i>Ing3</i> injection on asymmetric cell division in mouse oocytes.

<p>(A) <i>Ing3</i> mRNA was significantly decreased in the fully grown GV oocytes injected with si<i>Ing3</i> (*<i>p</i><0.05). (B) ING3 protein levels, after normalization to α-tubulin, were decreased in fully grown GV oocytes injected with si<i>Ing3</i>. (C) Abnormal cell division was observed in several si<i>Ing3</i>-injected oocytes at the MII stage (arrows). (D) In si<i>Ing3</i>-injected oocytes, the rate of symmetric division was significantly increased as compared to that observed in siControl-injected oocytes (*<i>p</i><0.05). (E) Maturation rates were not different between si<i>Ing3</i>- and siControl-injected oocytes.</p

Risk of ventilator-associated conditions: VAC using Cox proportional hazard model (Stepwise Variable Selection) (n = 303).

<p>Risk of ventilator-associated conditions: VAC using Cox proportional hazard model (Stepwise Variable Selection) (n = 303).</p

Effects of si<i>Ing3</i> injection on cortical reorganization in mouse oocytes.

<p>(A) Actin cap formation was noted in siControl-injected oocytes at the MI, ATI, and MII stages. By contrast, no actin cap was formed in si<i>Ing3</i>-injected oocytes at any stage. Arrows indicate the actin cap. Green, α-tubulin; red, actin; blue, chromatin. (B) CGs were absent in the cortex where the chromosomes were located at the MI and MII stages in siControl-injected oocytes. By contrast, in si<i>Ing3</i>-injected oocytes, CGs were distributed throughout the cortex at the MI and MII stages, and were intensely localized in the region of cell adhesion at the MII stage. Circles and arrows denote the CGFD. Green, cortical granules; blue, chromatin.</p

Localization of ING3 during mouse oocyte maturation.

<p>(A) ING3 was predominantly localized in the nucleus in fully grown GV oocytes. After GVBD, ING3 localized homogeneously throughout the cytoplasm. Green, ING3; blue, chromatin. (B) ING3 bound to the chromatin in fully grown GV oocytes. Green, ING3; blue, chromatin.</p

Patient disposition chart.

<p>CDC, Centers for Disease Control and Prevention; ECMO, extracorporeal membrane oxygenation; ICU, intensive-care unit; MV, mechanical ventilation; PCPS, percutaneous cardiopulmonary support; NPPV, noninvasive positive pressure ventilation; VAC, ventilator-associated conditions</p

Baseline demographics and outcome.

<p>Baseline demographics and outcome.</p

Ventilator-associated conditions univariate analysis.

<p>Ventilator-associated conditions univariate analysis.</p

Aggregation of pnbiPSCs into <i>in vitro</i> fertilized embryos, and their development <i>in vitro</i>.

<p>*RFP vector was introduced</p><p>Aggregation of pnbiPSCs into <i>in vitro</i> fertilized embryos, and their development <i>in vitro</i>.</p